Selected article for: "chain reaction and rt PCR chain reaction"
Author: Jung, Juwon; Baek, Jin Ah; Seol, Hye Won; Choi, Young Min
Title: Propagation of Human Embryonic Stem Cells on Human Amniotic Fluid Cells as Feeder Cells in Xeno-Free Culture Conditions
  • Document date: 2016_3_23
    • ID: 20xe4vm6_8
    Snippet: In order to examine whether the hESCs cultured using amniotic fluid cells as feeder cells maintained their own properties, immunocytochemistry (ICC), reverse transcription polymerase chain reaction (RT-PCR), and flow cytometry were used to analyze expression/activity of alkaline phosphatase (AP), a cell surface marker for the undifferentiated state of hESCs, and the expression of pluripotency markers, i.e., Oct-4, SSEA-4, TRA1-60 and TRA1-81......
    Document: In order to examine whether the hESCs cultured using amniotic fluid cells as feeder cells maintained their own properties, immunocytochemistry (ICC), reverse transcription polymerase chain reaction (RT-PCR), and flow cytometry were used to analyze expression/activity of alkaline phosphatase (AP), a cell surface marker for the undifferentiated state of hESCs, and the expression of pluripotency markers, i.e., Oct-4, SSEA-4, TRA1-60 and TRA1-81.

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