Quantitative Proteomic Analysis of A549 Cells Infected with Human Respiratory Syncytial Virus

Munday, Diane C.; Emmott, Edward; Surtees, Rebecca; Lardeau, Charles-Hugues; Wu, Weining; Duprex, W. Paul; Dove, Brian K.; Barr, John N.; Hiscox, Julian A.

Pathway analysis was used to group proteins into different functional networks to determine whether different cellular activities were altered in HRSV-infected cells. For example, in the nuclear fraction, proteins involved in cell growth and transcription regulation (Fig. 3) were less abundant in HRSVinfected cells. Such pathways were linked by cell cycle regulatory complexes (e.g. cyclin A) and IFN ␤, which were not identified in the LC-MS/MS analysis. Additionally, in the nuclear fraction, proteins involved in molecular transport and protein and RNA trafficking could be grouped together, and all were less abundant in the nucleus in HRSV-infected cells (Fig. 4) . In the cytoplasmic fraction, proteins involved in cellular assembly and organization, cellular compromise, and protein folding were grouped together. Proteins could be linked by NF-B and STAT1 signaling for example (Fig. 5) .