Quantitative Proteomic Analysis of A549 Cells Infected with Human Respiratory Syncytial Virus

Munday, Diane C.; Emmott, Edward; Surtees, Rebecca; Lardeau, Charles-Hugues; Wu, Weining; Duprex, W. Paul; Dove, Brian K.; Barr, John N.; Hiscox, Julian A.

Validation of IPA Analysis for HRSV-infected Cells Using Previously Characterized Immune Signaling-The HRSV-immune response interaction has been well characterized in vitro and in vivo (e.g. Ref. 20) . In the quantitative proteomics analysis of A549 cells infected with HRSV, STAT1 and its downstream molecule IFN-stimulated gene 15 protein (ISG15) were more abundant than in mock-infected cells (Fig. 5, left) . This observation reflects previous work in which human diploid fibroblast 2fTGH cells were infected with HRSV, and STAT1 protein was shown to be more abundant in HRSVinfected cells when compared with mock-infected cells (43) . The role of STAT1 in the immunobiology of HRSV has been investigated in transgenic animal models (44) . The IFN-stimulated gene 15 mRNA and protein were shown to be upregulated in a mouse lung epithelial cell line (MLE-15) infected with HRSV (45) . In the current data set, these molecules were linked to NF-B-activated transcription, transforming growth factor ␤1, and IFN ␣/␤ (Fig. 5) , all of which have been described in HRSV-infected cells (5, 18, 20, 21, 44, 46, 47) . Therefore, previously published data were reflected by the bioinformatics analysis of the current quantitative proteomics data.