Quantitative Proteomic Analysis of A549 Cells Infected with Human Respiratory Syncytial Virus

Munday, Diane C.; Emmott, Edward; Surtees, Rebecca; Lardeau, Charles-Hugues; Wu, Weining; Duprex, W. Paul; Dove, Brian K.; Barr, John N.; Hiscox, Julian A.

Disruption to Subnuclear Structures, ND10s (PML Bodies), and Associated Proteins-Many subnuclear structures such as the nucleolus and ND10s (also known as PML bodies) contain proteins that are involved in the antiviral signaling response that become altered in virus-infected cells (61) (62) (63) . The nucleolus is formed from a complex of protein-protein and protein-nucleic acid interactions centered around nucleolar hub proteins such as nucleolin and nucleophosmin (64) . ND10s are formed around PML protein and are dynamic structures that are in continuous protein exchange with the nucleus, depending on the metabolic state of the cell (65) . PML is a protein with several different isoforms that can localize to ND10s and/or the nucleus or cytoplasm (66) . Several components of the subnuclear structures were altered in HRSV-infected cells, including an ablation of nucleolin (decreased 4.5-fold) and nucleophosmin (decreased 6.7-fold) (see supplemental Table 1 ; for nucleolin, shown using Western blot, see Fig. 11 ).