Author: Prerana Shrestha; Pinar Ayata; Pedro Herrero-Vidal; Francesco Longo; Alexandra Gastone; Joseph E. Ledoux; Nathaniel Heintz; Eric Klann
Title: Chemogenetic evidence that rapid neuronal de novo protein synthesis is required for consolidation of long-term memory Document date: 2019_7_17
ID: llzdwza1_5
Snippet: To test the efficiency of ciPSI in blocking protein synthesis ex vivo, amygdala slices of Nes.iPKR mice 168 and wild-type (WT) mice were subjected to bio-orthogonal non-canonical amino-acid tagging (BONCAT) 27 surface sensing of translation (SUnSET) 28 , that measures translation elongation and is amenable for in vivo 174 labeling, was used to test ciPSI efficiency in awake behaving mice. SUnSET immunoblot showed that 175 Nes.iPKR mice centrally .....
Document: To test the efficiency of ciPSI in blocking protein synthesis ex vivo, amygdala slices of Nes.iPKR mice 168 and wild-type (WT) mice were subjected to bio-orthogonal non-canonical amino-acid tagging (BONCAT) 27 surface sensing of translation (SUnSET) 28 , that measures translation elongation and is amenable for in vivo 174 labeling, was used to test ciPSI efficiency in awake behaving mice. SUnSET immunoblot showed that 175 Nes.iPKR mice centrally infused with 150 pg ASV exhibited a robust decrease in protein synthesis (~50%) 176 compared to controls (Fig. 2b) . The inhibition of protein synthesis was concomitant with a specific increase in 177 phosphorylation of eIF2α at 1h post ASV treatment both ex vivo (Fig. 2c ) and in vivo (Fig. 2d) , with no effect 178 on either the extracellular signal regulated kinase (ERK1/2) or mechanistic target of rapamycin (mTORC1) 179 pathways (Fig. 2d ). Next, we investigated the ASV pharmacokinetics in Nes.iPKR amygdala lysates by 180 harvesting tissue at increasing time points after drug infusion. We found that peak expression of iPKR is 181 reached at 0.5h, which steadily declines at 1h and 3h and is completely degraded by 6h (Fig. 2e) .
Search related documents:
Co phrase search for related documents- amino acid and extracellular signal: 1, 2, 3, 4
- amino acid tagging and BONCAT amino acid tagging: 1
Co phrase search for related documents, hyperlinks ordered by date