Selected article for: "room temperature and soybean trypsin"
Author: Qiao, Hui; Pelletier, Sandra L.; Hoffman, Lucas; Hacker, Jill; Armstrong, R. Todd; White, Judith M.
Title: Specific Single or Double Proline Substitutions in the “Spring-loaded” Coiled-Coil Region of the Influenza Hemagglutinin Impair or Abolish Membrane Fusion Activity
  • Document date: 1998_6_15
    • ID: 78fjem8s_12
    Snippet: As indicated, cells expressing wt-or mutant HAs were washed twice with RPMI or PBS and incubated for 6 min at room temperature (RT) with RPMI or PBS containing either TPCK-trypsin (5 g/ml) or, as a negative control, TLCK-chymotrypsin (5 g/ml) (Sigma Chemical Co., St. Louis, MO). Trypsin-treated cells were then incubated for 10 min with RPMI or PBS containing soybean trypsin inhibitor (50 g/ml). Higher concentrations of trypsin (10 g/ml) were used.....
    Document: As indicated, cells expressing wt-or mutant HAs were washed twice with RPMI or PBS and incubated for 6 min at room temperature (RT) with RPMI or PBS containing either TPCK-trypsin (5 g/ml) or, as a negative control, TLCK-chymotrypsin (5 g/ml) (Sigma Chemical Co., St. Louis, MO). Trypsin-treated cells were then incubated for 10 min with RPMI or PBS containing soybean trypsin inhibitor (50 g/ml). Higher concentrations of trypsin (10 g/ml) were used where noted to increase the amount of cleaved HA at the cell surface.

    Search related documents:
    Co phrase search for related documents
    • cell surface and mutant wt: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • cell surface and negative control: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • cell surface and room temperature: 1, 2, 3, 4, 5, 6
    • cleave HA and mutant wt: 1
    • high concentration and mutant wt: 1
    • high concentration and negative control: 1, 2
    • high concentration and room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • high concentration and RT room temperature: 1
    • mutant wt and negative control: 1, 2, 3, 4, 5, 6
    • mutant wt and room temperature: 1
    • negative control and room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
    • negative control and twice wash: 1, 2
    • room temperature and RT room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46
    • room temperature and twice wash: 1, 2, 3, 4, 5