Selected article for: "cacodylate buffer and electron microscope"
Title: Effect of caffeine and reduced temperature (20 degrees C) on the organization of the pre-Golgi and the Golgi stack membranes
  • Document date: 1993_3_2
    • ID: 7c7slfbp_18
    Snippet: In conventional electron microscopy the cells were fixed for 1 h at room temperature with a fixative containing 2 % glutaraldehyde and 3 % paraformaldehyde in 100 mM cacodylate buffer, pH 7.4, and postfixed with reduced 1% OsO4 for 1 h on ice. All samples were embedded in LX-112 (Ladd Research Industries Inc., Burlington, VT) and were viewed with a Jeol JEM-1200EX transmission electron microscope operated at an acceleration voltage of 60 kV......
    Document: In conventional electron microscopy the cells were fixed for 1 h at room temperature with a fixative containing 2 % glutaraldehyde and 3 % paraformaldehyde in 100 mM cacodylate buffer, pH 7.4, and postfixed with reduced 1% OsO4 for 1 h on ice. All samples were embedded in LX-112 (Ladd Research Industries Inc., Burlington, VT) and were viewed with a Jeol JEM-1200EX transmission electron microscope operated at an acceleration voltage of 60 kV.

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