Effect of caffeine and reduced temperature (20 degrees C) on the organization of the pre-Golgi and the Golgi stack membranes

The antibodies were kind gifts from the following persons: polyclonal antibodies to/S-COP (EAGE) (Duden et al., 1991) were from Drs. R. Duden (EMBL, Heidelberg, Germany) and T. Kreis (University of Geneva, Geneva, Switzerland); polyclonal antibodies to man II were from Drs. M. Farquhar (University of California, San Diego, CA) and K. Moremen (Massachusetts Institute of Technology, Cambridge, MA); monoclonal antibodies to man II (135-kD protein) were from Drs. B. Burke (Harvard Medical School, Boston, MA) and G. Warren (Imperial Cancer Research Fund, London, UK) (Burke et al., 1982) ; polyclonal antibodies to p58 were from Dr. J. Saraste (University of Bergen, Bergen, Norway) (Saraste and Svensson, 1991) ; and polyclonal antibodies to SFV glycoproteins El and E2 were from Dr. J. Pe~nen (University of Helsinki, Helsinki, Finland).