Author: Dong, Xiaoxv; Ni, Boran; Fu, Jing; Yin, Xingbin; You, Longtai; Leng, Xin; Liang, Xiao; Ni, Jian
Title: Emodin induces apoptosis in human hepatocellular carcinoma HepaRG cells via the mitochondrial caspase-dependent pathway Document date: 2018_8_2
ID: 02r8i5n1_11
Snippet: Cell cycle analysis. HepaRG cells (4.0x10 5 cells/well) were incubated in 6-well plates and treated with different concentrations of emodin (20, 40 and 80 µM) for 24 h at 37˚C. After treatment, the cells were collected and fixed with 70% ice-cold ethanol at 4˚C at least for 24 h, and then stained with PI/RNase A staining buffer solution for 30 min at 37˚C in the dark (36) . The resulting suspension was passed through a nylon mesh filter and a.....
Document: Cell cycle analysis. HepaRG cells (4.0x10 5 cells/well) were incubated in 6-well plates and treated with different concentrations of emodin (20, 40 and 80 µM) for 24 h at 37˚C. After treatment, the cells were collected and fixed with 70% ice-cold ethanol at 4˚C at least for 24 h, and then stained with PI/RNase A staining buffer solution for 30 min at 37˚C in the dark (36) . The resulting suspension was passed through a nylon mesh filter and analyzed by flow cytometry.
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