Selected article for: "ER localization and Golgi localization"
Title: Endoplasmic reticulum localization of Sec12p is achieved by two mechanisms: Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain
  • Document date: 1996_7_2
    • ID: 45x96b5d_26
    Snippet: Since SSSm is localized to the ER, whereas DDDm is transported through the late Golgi to the vacuole, we went on to ask, in Sec12/Dap2-Mfodp chimeric proteins, which part of Sec12p could retain the whole molecule in the ER or the early Golgi? This was tested by performing halo assays on Mfalp fusions with all six chimeras we constructed, The fusion proteins were named with three letters composed of S and/or D followed by m (Fig. 1) . As Rerlp pla.....
    Document: Since SSSm is localized to the ER, whereas DDDm is transported through the late Golgi to the vacuole, we went on to ask, in Sec12/Dap2-Mfodp chimeric proteins, which part of Sec12p could retain the whole molecule in the ER or the early Golgi? This was tested by performing halo assays on Mfalp fusions with all six chimeras we constructed, The fusion proteins were named with three letters composed of S and/or D followed by m (Fig. 1) . As Rerlp plays an important role in the ER localization of Sec12p, probably in the retrieval from the Golgi, all the experiments were done for both wild-type (SNY9) and

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