Selected article for: "electron density and final model"
Author: Deng, Zengqin; Lehmann, Kathleen C.; Li, Xiaorong; Feng, Chong; Wang, Guoqiang; Zhang, Qi; Qi, Xiaoxuan; Yu, Lin; Zhang, Xingliang; Feng, Wenhai; Wu, Wei; Gong, Peng; Tao, Ye; Posthuma, Clara C.; Snijder, Eric J.; Gorbalenya, Alexander E.; Chen, Zhongzhou
Title: Structural basis for the regulatory function of a complex zinc-binding domain in a replicative arterivirus helicase resembling a nonsense-mediated mRNA decay helicase
  • Document date: 2013_12_24
    • ID: 471zei5o_14
    Snippet: Using the structure of free nsp10Á without domain 1B as input model, the structure of nsp10Á in complex with DNA was successfully solved by molecular replacement. The initial model was obtained by MOLREP from the CCP4 program suite (46) . A good match for domains ZBD, 1A and 2A with electron density was found. Domain 1B was manually added with the aid of 2F o -F c and F o -F c maps using COOT (43) . DNA molecules were included in the final stag.....
    Document: Using the structure of free nsp10Á without domain 1B as input model, the structure of nsp10Á in complex with DNA was successfully solved by molecular replacement. The initial model was obtained by MOLREP from the CCP4 program suite (46) . A good match for domains ZBD, 1A and 2A with electron density was found. Domain 1B was manually added with the aid of 2F o -F c and F o -F c maps using COOT (43) . DNA molecules were included in the final stages of refinement. Difference Fourier maps clearly showed electron densities for seven bound deoxyribonucleotides. The final model was refined to 2.65 Å with an R work of 23.2% and an R free of 25.7%. All figures in this article displaying molecular structure were made using PYMOL (47) .

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