Author: Srivastava, Sukrit; Kamthania, Mohit; Singh, Soni; Saxena, Ajay K; Sharma, Nishi
Title: Structural basis of development of multi-epitope vaccine against Middle East respiratory syndrome using in silico approach Document date: 2018_11_21
ID: 33h22ikl_6
Snippet: structural basis of development of multi-epitope vaccine against MeRs (3CLpro); ORF1ab (4WUR), a PL(pro); ORF1ab (5WWP); and ORF8b. For sequence-based epitope screening, fulllength protein sequences of the abovementioned MERS-CoV proteins were retrieved from the NCBI database (National Center for Biotechnology Information, https://www.ncbi. nlm.nih.gov/protein). A total of 2,499 amino acid sequences belonging to different proteins of MERS-CoV of .....
Document: structural basis of development of multi-epitope vaccine against MeRs (3CLpro); ORF1ab (4WUR), a PL(pro); ORF1ab (5WWP); and ORF8b. For sequence-based epitope screening, fulllength protein sequences of the abovementioned MERS-CoV proteins were retrieved from the NCBI database (National Center for Biotechnology Information, https://www.ncbi. nlm.nih.gov/protein). A total of 2,499 amino acid sequences belonging to different proteins of MERS-CoV of different strains and origins were retrieved. For structure-based epitope screening, available 3D structures of MERS-CoV proteins were retrieved from Protein Data Bank (PDB, http://www.rcsb.org/pdb/home/home.do). For the proteins with no structure available in PDB, homology modeling was performed using the Swiss-model, (http://swissmodel. expasy.org/) (Table S1 ). 22
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