Selected article for: "ER return and mannosyl modification"

Title: Endoplasmic reticulum localization of Sec12p is achieved by two mechanisms: Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain
  • Document date: 1996_7_2
  • ID: 45x96b5d_44
    Snippet: SDD was detected as a 152-kD band (Fig. 9 B) . In contrast to DSD, the rate of od--->6 mannosyl modification on SDD was very slow. Only 19% was modified with the cd---~6 linkage after 60 min, which is very similar to the case of Secl2p (see Table III ). It appears that the acquisition of the ctl-->3 modification of SDD (8% at 60 min) is significantly faster than either Secl2p or DSD. Since the ER localization of SDD does not require Rerlp, which .....
    Document: SDD was detected as a 152-kD band (Fig. 9 B) . In contrast to DSD, the rate of od--->6 mannosyl modification on SDD was very slow. Only 19% was modified with the cd---~6 linkage after 60 min, which is very similar to the case of Secl2p (see Table III ). It appears that the acquisition of the ctl-->3 modification of SDD (8% at 60 min) is significantly faster than either Secl2p or DSD. Since the ER localization of SDD does not require Rerlp, which is involved in the retrieval, it may be reasonable that the SDD molecules are not sent back to the ER efficienctly once they have left. In fact, the immunofluorescence of SDD (see Fig. 6 D) indicates that some population of SDD is transported to the vacuole. The results of the halo assay on SDDm also supports this. All these observations are consistent with the idea that SDD is localized to the ER by moderate static retention. A small portion escapes from this mechanism, and the molecules that depart from the ER do not return but reach the vacuole.

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