Selected article for: "clone plasmid and copy number"
Author: Almazán, Fernando; DeDiego, Marta L.; Sola, Isabel; Zuñiga, Sonia; Nieto-Torres, Jose L.; Marquez-Jurado, Silvia; Andrés, German; Enjuanes, Luis
Title: Engineering a Replication-Competent, Propagation-Defective Middle East Respiratory Syndrome Coronavirus as a Vaccine Candidate
  • Document date: 2013_9_10
    • ID: 14yfs4pa_33
    Snippet: Plasmids and bacteria strains. Plasmid pBeloBAC11 (61), kindly provided by H. Shizuya (California Institute of Technology, Pasadena, CA), was used to assemble the MERS-CoV infectious cDNA clone. This plasmid is a low-copy-number plasmid (one to two copies per cell) based on the E. coli F factor (62) that allows the stable maintenance of large DNA fragments in bacteria. E. coli DH10B (Gibco/BRL) cells were transformed by electroporation using a Mi.....
    Document: Plasmids and bacteria strains. Plasmid pBeloBAC11 (61), kindly provided by H. Shizuya (California Institute of Technology, Pasadena, CA), was used to assemble the MERS-CoV infectious cDNA clone. This plasmid is a low-copy-number plasmid (one to two copies per cell) based on the E. coli F factor (62) that allows the stable maintenance of large DNA fragments in bacteria. E. coli DH10B (Gibco/BRL) cells were transformed by electroporation using a MicroPulser unit (Bio-Rad) according to the manufacturer's instructions. BAC plasmid and recombinant BACs were isolated and purified using a large-construct kit (Qiagen), following the manufacturer's specifications.

    Search related documents:
    Co phrase search for related documents
    • BAC plasmid and cell copy: 1
    • BAC plasmid and copy number: 1, 2
    • BAC plasmid and infectious cdna clone: 1
    • bacteria dna fragment and dna fragment: 1
    • cdna clone and cell copy: 1
    • cdna clone and copy number: 1, 2, 3, 4, 5
    • cdna clone and dh10b coli: 1
    • cdna clone and dna fragment: 1, 2, 3, 4, 5
    • cdna clone and infectious cdna clone: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • cdna clone and large construct: 1
    • cell copy and copy number: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
    • cell copy and infectious cdna clone: 1
    • copy number and dh10b coli: 1
    • copy number and dna fragment: 1, 2
    • copy number and infectious cdna clone: 1, 2
    • dh10b coli and infectious cdna clone: 1
    • dna fragment and infectious cdna clone: 1, 2
    • dna fragment and large construct: 1
    • infectious cdna clone and large construct: 1