Selected article for: "blot analysis and expression level"
Author: Mohammadzadeh, Sara; Khabiri, Alireza; Roohvand, Farzin; Memarnejadian, Arash; Salmanian, Ali Hatef; Ajdary, Soheila; Ehsani, Parastoo
Title: Enhanced-Transient Expression of Hepatitis C Virus Core Protein in Nicotiana tabacum, a Protein With Potential Clinical Applications
  • Document date: 2014_11_24
    • ID: 3posyr5n_23
    Snippet: To detect and analyze pHCVcp, the transformed tobacco leave extracts were evaluated by dot and western blot assays ( Figure 4 ). As shown in Figure 4 A, dot blot analysis showed that HCV core was present in extracts of the tobacco leaves, transformed by each of the vectors (pBI-core and PVX-core), compared to negative controls with the same amount of loaded TSP. Although dot blot is a qualitative assay rather than a quantitative one, our result t.....
    Document: To detect and analyze pHCVcp, the transformed tobacco leave extracts were evaluated by dot and western blot assays ( Figure 4 ). As shown in Figure 4 A, dot blot analysis showed that HCV core was present in extracts of the tobacco leaves, transformed by each of the vectors (pBI-core and PVX-core), compared to negative controls with the same amount of loaded TSP. Although dot blot is a qualitative assay rather than a quantitative one, our result tentatively implied a higher level of expression for PVX-core compared with that of pBI-core construct, while the same amounts of TSP were applied (Figure 4 A) .

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