Author: Mateo, Roberto; Nagamine, Claude M.; Kirkegaard, Karla
Title: Suppression of Drug Resistance in Dengue Virus Document date: 2015_12_15
ID: 6bx2nrui_15
Snippet: To ask whether ST-148-resistant viruses could be selected during murine infection, C57BL/6 IFNAR Ϫ/Ϫ IFNGR Ϫ/Ϫ mice were inoculated with dengue virus and treated orally with ST-148, or vehicle solution as a control, twice daily for 4 days as described previously (12) . Treatment with ST-148 led to a significant decrease in dengue virus yield (Fig. 5B) . To detect the emergence of drug resistance, spleen homogenates from control and ST-148trea.....
Document: To ask whether ST-148-resistant viruses could be selected during murine infection, C57BL/6 IFNAR Ϫ/Ϫ IFNGR Ϫ/Ϫ mice were inoculated with dengue virus and treated orally with ST-148, or vehicle solution as a control, twice daily for 4 days as described previously (12) . Treatment with ST-148 led to a significant decrease in dengue virus yield (Fig. 5B) . To detect the emergence of drug resistance, spleen homogenates from control and ST-148treated mice were diluted and used to infect fresh monolayers of BHK-21 cells in the presence of ST-148 (Fig. 5A) . After 2 days, supernatant titers were determined and RNA was quantified. Strikingly, no amplification of ST-148-resistant virus (Fig. 5C ) or of RNA populations (Fig. 5D ) was observed.
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