Author: Mateo, Roberto; Nagamine, Claude M.; Kirkegaard, Karla
Title: Suppression of Drug Resistance in Dengue Virus Document date: 2015_12_15
ID: 6bx2nrui_33
Snippet: Cell fractionation, protein precipitation, and quantitation. Fractionation of cultured cells was performed using the Qproteome cell fractionation kit (Qiagen, Valencia, CA) following the manufacturer's instructions. Proteins from each fraction were methanol precipitated, resuspended in 8 M urea, 100 mM Tris-HCl buffer (pH 8.8), and quantified using the DC protein assay kit (Bio-Rad, Hercules, CA)......
Document: Cell fractionation, protein precipitation, and quantitation. Fractionation of cultured cells was performed using the Qproteome cell fractionation kit (Qiagen, Valencia, CA) following the manufacturer's instructions. Proteins from each fraction were methanol precipitated, resuspended in 8 M urea, 100 mM Tris-HCl buffer (pH 8.8), and quantified using the DC protein assay kit (Bio-Rad, Hercules, CA).
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