Selected article for: "co expression and gene expression"
Author: Mohammadzadeh, Sara; Khabiri, Alireza; Roohvand, Farzin; Memarnejadian, Arash; Salmanian, Ali Hatef; Ajdary, Soheila; Ehsani, Parastoo
Title: Enhanced-Transient Expression of Hepatitis C Virus Core Protein in Nicotiana tabacum, a Protein With Potential Clinical Applications
  • Document date: 2014_11_24
    • ID: 3posyr5n_35
    Snippet: For obtaining the highest expression levels, it is important to prevent the silencing mechanisms that limit foreign gene expressions in plants. It is shown that coexpression of viral silencing suppressor proteins eliminates the effects of post-transcriptional gene silencing (PTGS) in a transient expression system (42) . Application of P19-mediated co-agroinfiltration has already shown to improve recombinant expression in plants by several folds (.....
    Document: For obtaining the highest expression levels, it is important to prevent the silencing mechanisms that limit foreign gene expressions in plants. It is shown that coexpression of viral silencing suppressor proteins eliminates the effects of post-transcriptional gene silencing (PTGS) in a transient expression system (42) . Application of P19-mediated co-agroinfiltration has already shown to improve recombinant expression in plants by several folds (42) . Our results indicated that the presence of P19 increased the expression levels of pHCVcp by five times in both pBI121 and PVX expression constructs (Figure 5 A) . Our data were consistent with recent reports on enhancement of the expression levels of SARS-CoV N (54), antibodies (53) and GFP (55) proteins in the presence of P19 by 3-5 and even 100 folds, respectively.

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