Title: Enhancement of IgE-mediated histamine release from human basophils by viruses: role of interferon Document date: 1977_4_1
ID: 5v7k90zr_4
Snippet: Histamine Release Reaction. Leukocyte suspensions, containing about 5 x 10 s cells in 0.5 ml, were incubated with either an equal volume of infectious virus or the equivalent amount of UVinactivated virus or the appropriate control media. Except where stated, a virus:cell ratio of 1:1, expressed as the multiplicity of infection (moi), was used. After incubation at 37°C in a 5% COs atmosphere for 2 h, the cells were washed, resuspended in RPMI me.....
Document: Histamine Release Reaction. Leukocyte suspensions, containing about 5 x 10 s cells in 0.5 ml, were incubated with either an equal volume of infectious virus or the equivalent amount of UVinactivated virus or the appropriate control media. Except where stated, a virus:cell ratio of 1:1, expressed as the multiplicity of infection (moi), was used. After incubation at 37°C in a 5% COs atmosphere for 2 h, the cells were washed, resuspended in RPMI medium, and incubated for an additional 22 h at 37°C. All experiments were performed at least twice and in each experiment reactions were set up in duplicate. Controls consisted of media, uninfected PRK cells, or allantoic fluid prepared and diluted in the same manner as the virus pools.
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