Selected article for: "quantitative polymerase chain reaction and real time"
Author: Baek, Hyekyung; Kim, Kwang Ho; Park, Min Young; Kim, Kyeongryun; Ko, Bokyeong; Seo, Hyung Seok; Kim, Byoung Soo; Hahn, Tae-Wook; Yi, Sun Shin
Title: Establishment of minimal positive-control conditions to ensure brain safety during rapid development of emergency vaccines
  • Document date: 2017_8_22
    • ID: 52sbckm3_24
    Snippet: How to apply the lipopolysaccharide (LPS) positive-control protocol to verify vaccine safety at the blood-brain barrier (BBB). Based on the results of our experiments, we have established a positive-control protocol for determining vaccine safety at the BBB. Salmonella enterica-derived LPS and vaccine are intraperitoneally injected into 7-week-old ICR mice (1.0 mg LPS/4 mL PBS/kg body weight) and brain hemispheres are removed after 4 h. The mRNA .....
    Document: How to apply the lipopolysaccharide (LPS) positive-control protocol to verify vaccine safety at the blood-brain barrier (BBB). Based on the results of our experiments, we have established a positive-control protocol for determining vaccine safety at the BBB. Salmonella enterica-derived LPS and vaccine are intraperitoneally injected into 7-week-old ICR mice (1.0 mg LPS/4 mL PBS/kg body weight) and brain hemispheres are removed after 4 h. The mRNA is extracted, followed by quantitative real-time polymerase chain reaction (PCR) using zonula occludens-1 (ZO-1), occludin, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) primers. By comparing the tight junction mRNA levels of the vaccine-injected group with those of the LPS-injected group, the potential danger to the BBB of the vaccine is evaluated.

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