Establishment of minimal positive-control conditions to ensure brain safety during rapid development of emergency vaccines

Baek, Hyekyung; Kim, Kwang Ho; Park, Min Young; Kim, Kyeongryun; Ko, Bokyeong; Seo, Hyung Seok; Kim, Byoung Soo; Hahn, Tae-Wook; Yi, Sun Shin

In order to investigate the mouse strain-based differences in the participating BBB structural disruption mechanisms, further protein analyses such as those in proteomic studies should be considered. The relationships between mRNA levels and protein expressions of ZO-1 or occludin in ICR mice are approximated in panel C in Fig. 4 , which includes arrows showing translational streams. While ZO-1 mRNA levels in ICR BBB increased until 4 h PI and decreased until 24 h PI, the ZO-1 protein expression was continuously increased until 24 h PI. This time lag is thought to be caused by the process requirements for protein synthesis from mRNA and by the protein expression regulatory activity of the biological mechanism within brain epithelial cells. The expression of occludin mRNA and protein were rather more slowly increased than those of ZO-1. The location of proteins and accessibility of proteasomes for occludin as a transmembrane protein and for ZO-1 as an intracellular protein might be factors in the temporal differences between the two TJ proteins.