Title: The amino-terminal domain of the lamin B receptor is a nuclear envelope targeting signal Document date: 1993_3_1
ID: 377v2ufn_10
Snippet: Complementary DNA coding for chimpanzee c~-globin was cloned into pSVK3 to generate plasmid AG. A plasmid containing a cDNA insert coding for chimpanzee c~-globin (Rothman et al., 1988 ) was a gift from Dr. V. R. Lingappa (University of California, San Francisco). This plasmid was used as template for PCR to generate cr cDNA from the initiator ATG through the TAA stop codon (cDNA sequence according to Liebhaber and Begley, 1984) . The sense prime.....
Document: Complementary DNA coding for chimpanzee c~-globin was cloned into pSVK3 to generate plasmid AG. A plasmid containing a cDNA insert coding for chimpanzee c~-globin (Rothman et al., 1988 ) was a gift from Dr. V. R. Lingappa (University of California, San Francisco). This plasmid was used as template for PCR to generate cr cDNA from the initiator ATG through the TAA stop codon (cDNA sequence according to Liebhaber and Begley, 1984) . The sense primer had an EcoRI site engineered into its 5' end and the antisense primer a Sail site at its 5' end. The product was purified from the reaction mixture by phenol:chloroform extraction, digested with EcoRI and Sail, purified again and ligated into the EcoRI/SalI site of pSVK3.
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