Title: The amino-terminal domain of the lamin B receptor is a nuclear envelope targeting signal Document date: 1993_3_1
ID: 377v2ufn_14
Snippet: COS-7 cells were grown in DME media containing 10% FBS, 50 U/ml penicillin, 50 #g/ml streptomycin and 2 mM I-glutamine. For transfection, cells were grown to 60 % confluency on 60-ram-diameter petri dishes. Cells were transfected by the calcium precipitation method as described (Sambrook et al., 1989) . The precipitated plasmid DNA (10/xg) was left on each petri dish for 5 h. Ceils were then washed with PBS, split into 35-ram-diameter petri dishe.....
Document: COS-7 cells were grown in DME media containing 10% FBS, 50 U/ml penicillin, 50 #g/ml streptomycin and 2 mM I-glutamine. For transfection, cells were grown to 60 % confluency on 60-ram-diameter petri dishes. Cells were transfected by the calcium precipitation method as described (Sambrook et al., 1989) . The precipitated plasmid DNA (10/xg) was left on each petri dish for 5 h. Ceils were then washed with PBS, split into 35-ram-diameter petri dishes and maintained in culture for 12 to 72 h before fixation for immunofluorescence microscopy.
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