Selected article for: "antisense primer end and PCR product"
Title: The amino-terminal domain of the lamin B receptor is a nuclear envelope targeting signal
  • Document date: 1993_3_1
    • ID: 377v2ufn_7
    Snippet: Plasmid AT was constructed to express the amino-terminal domain of chicken LBR. A PCR product from nucleotides -42 to +625 of LBR cDNA was amplified using clone DJ-5 as template. The sense primer had an EcoRI site at the 5' end and the antisense primer a complementary stop codon preceZ M by a SalI site at its 5' end. The amplified product was purified from the reaction mixture using GeneClean, digested with EcoRI and SalI, purified again and liga.....
    Document: Plasmid AT was constructed to express the amino-terminal domain of chicken LBR. A PCR product from nucleotides -42 to +625 of LBR cDNA was amplified using clone DJ-5 as template. The sense primer had an EcoRI site at the 5' end and the antisense primer a complementary stop codon preceZ M by a SalI site at its 5' end. The amplified product was purified from the reaction mixture using GeneClean, digested with EcoRI and SalI, purified again and ligated into the EcoRI/SalI site of pSVK3.

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