Author: MINAMI, Shohei; TERADA, Yutaka; SHIMODA, Hiroshi; TAKIZAWA, Masaki; ONUMA, Mamoru; OTA, Akihiko; OTA, Yuichi; AKABANE, Yoshihito; TAMUKAI, Kenichi; WATANABE, Keiichiro; NAGANUMA, Yumiko; KANAGAWA, Eiichi; NAKAMURA, Kaneichi; OHASHI, Masanari; TAKAMI, Yoshinori; MIWA, Yasutsugu; TANOUE, Tomoaki; OHWAKI, Masao; OHTA, Jouji; UNE, Yumi; MAEDA, Ken
Title: Establishment of serological test to detect antibody against ferret coronavirus Document date: 2016_3_3
ID: 3g75spkc_2
Snippet: Although FRcoV genes were detected in ferrets by reverse transcription-polymerase chain reaction (RT-PcR), there is no method to detect antibodies to FRcoV. We attempted to isolate FRcoV using feline cell lines and our newly established ferret cell line (manuscript in preparation), but the virus has not yet been isolated. Because the nucleocapsid (N) is conserved between coronaviruses and used as an antigen to detect antibody [5, 8] , the N prote.....
Document: Although FRcoV genes were detected in ferrets by reverse transcription-polymerase chain reaction (RT-PcR), there is no method to detect antibodies to FRcoV. We attempted to isolate FRcoV using feline cell lines and our newly established ferret cell line (manuscript in preparation), but the virus has not yet been isolated. Because the nucleocapsid (N) is conserved between coronaviruses and used as an antigen to detect antibody [5, 8] , the N protein of FRcoV was one of the most likely antigen candidates to detect antibody to FRcoV. In this study, an enzyme-linked immunosorbent assay (ELISA) using recombinant N proteins was established and applied to investigate the seroprevalence of FRcoV infection in Japan.
Search related documents:
Co phrase search for related documents- antibody detect and chain reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
- antibody detect and ELISA immunosorbent assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
- antibody detect and immunosorbent assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20
- antibody detect method and immunosorbent assay: 1
- antigen candidate and cell line: 1, 2
- antigen candidate and chain reaction: 1
- antigen candidate and ELISA immunosorbent assay: 1, 2, 3, 4
- antigen candidate and immunosorbent assay: 1, 2, 3, 4, 5
- cell line and chain reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- cell line and ELISA immunosorbent assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
- cell line and feline cell line: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
- cell line and immunosorbent assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
- chain reaction and ELISA immunosorbent assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- chain reaction and enzyme link: 1
- chain reaction and ferret detect: 1
- chain reaction and immunosorbent assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- ELISA immunosorbent assay and enzyme link: 1
- ELISA immunosorbent assay and ferret detect: 1
- ELISA immunosorbent assay and FRcoV infection: 1
Co phrase search for related documents, hyperlinks ordered by date