Author: Sanz, Macarena G.; Kwon, SoYoung; Pusterla, Nicola; Gold, Jenifer R.; Bain, Fairfield; Evermann, Jim
Title: Evaluation of equine coronavirus fecal shedding among hospitalized horses Document date: 2019_2_20
ID: 6zomiz4g_8
Snippet: Electron microscopic evaluation was performed at the North Dakota State University Veterinary Diagnostic Laboratory, Fargo, North Dakota, as previously described. 16 Briefly, fecal material was mixed with 3-9 times its volume of deionized (DI) water to obtain the desired consistency. The suspension was allowed to settle and cleared by low-speed and then high-speed centrifugation. Pellets were resuspended in DI water, placed on 400-mesh, carbon-st.....
Document: Electron microscopic evaluation was performed at the North Dakota State University Veterinary Diagnostic Laboratory, Fargo, North Dakota, as previously described. 16 Briefly, fecal material was mixed with 3-9 times its volume of deionized (DI) water to obtain the desired consistency. The suspension was allowed to settle and cleared by low-speed and then high-speed centrifugation. Pellets were resuspended in DI water, placed on 400-mesh, carbon-stabilized grids and negatively stained for 30-60 seconds with 2% phosphotungstic acid solution. The grids were examined on a JEOL JEM-100CX II transmission electron microscope and evaluated by an experienced electron microscopist.
Search related documents:
Co phrase search for related documents- Electron microscopic evaluation and transmission electron: 1
- high speed and low speed: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49
- high speed and transmission electron: 1, 2, 3, 4, 5
- high speed and transmission electron microscope: 1
- microscopic evaluation and transmission electron: 1
- phosphotungstic acid and transmission electron: 1, 2, 3, 4, 5
- phosphotungstic acid and transmission electron microscope: 1, 2, 3
Co phrase search for related documents, hyperlinks ordered by date