Author: Sanz, Macarena G.; Kwon, SoYoung; Pusterla, Nicola; Gold, Jenifer R.; Bain, Fairfield; Evermann, Jim
Title: Evaluation of equine coronavirus fecal shedding among hospitalized horses Document date: 2019_2_20
ID: 6zomiz4g_8
Snippet: Electron microscopic evaluation was performed at the North Dakota State University Veterinary Diagnostic Laboratory, Fargo, North Dakota, as previously described. 16 Briefly, fecal material was mixed with 3-9 times its volume of deionized (DI) water to obtain the desired consistency. The suspension was allowed to settle and cleared by low-speed and then high-speed centrifugation. Pellets were resuspended in DI water, placed on 400-mesh, carbon-st.....
Document: Electron microscopic evaluation was performed at the North Dakota State University Veterinary Diagnostic Laboratory, Fargo, North Dakota, as previously described. 16 Briefly, fecal material was mixed with 3-9 times its volume of deionized (DI) water to obtain the desired consistency. The suspension was allowed to settle and cleared by low-speed and then high-speed centrifugation. Pellets were resuspended in DI water, placed on 400-mesh, carbon-stabilized grids and negatively stained for 30-60 seconds with 2% phosphotungstic acid solution. The grids were examined on a JEOL JEM-100CX II transmission electron microscope and evaluated by an experienced electron microscopist.
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