Selected article for: "ice 10 min and protease inhibitor"
Author: Mok, Hoyin; Cheng, Xing; Xu, Qi; Zengel, James R; Parhy, Bandita; Zhao, Jackie; Wang, C. Kathy; Jin, Hong
Title: Evaluation of Measles Vaccine Virus as a Vector to Deliver Respiratory Syncytial Virus Fusion Protein or Epstein-Barr Virus Glycoprotein gp350
  • Document date: 2012_2_16
    • ID: 3qdjmb2j_12
    Snippet: Western blot. Vero cells were infected at a MOI of 3.0 with rEZ, gp350, gp350tr, sF1 or sF3 for 18 hours at 37°C. Culture supernatant and cells were harvested separately. The cells were scraped and pelleted for 10 sec at 6,000 rpm in microfuge tubes and lysed in lysis buffer (50mM Tris-HCl, 150 mM NaCl, 1% Triton X-100, 0.5% [vol/vol] protease inhibitor cocktail, pH 8.0) (Sigma, St. Louis, MO) for 10 min on ice. The resulting cell lysates were c.....
    Document: Western blot. Vero cells were infected at a MOI of 3.0 with rEZ, gp350, gp350tr, sF1 or sF3 for 18 hours at 37°C. Culture supernatant and cells were harvested separately. The cells were scraped and pelleted for 10 sec at 6,000 rpm in microfuge tubes and lysed in lysis buffer (50mM Tris-HCl, 150 mM NaCl, 1% Triton X-100, 0.5% [vol/vol] protease inhibitor cocktail, pH 8.0) (Sigma, St. Louis, MO) for 10 min on ice. The resulting cell lysates were cleared by centrifugation at 13,000 rpm for 5 min.

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