Author: MYRRHA, Luciana Wanderley; SILVA, Fernanda Miquelitto Figueira; VIDIGAL, Pedro Marcus Pereira; RESENDE, Maurício; BRESSAN, Gustavo Costa; FIETTO, Juliana Lopes Rangel; SANTOS, Marcus Rebouças; SILVA, Laura Morais Nascimento; ASSAO, Viviane Sisdelli; SILVA-JÚNIOR, Abelardo; de ALMEIDA, Márcia Rogéria
Title: Feline coronavirus isolates from a part of Brazil: insights into molecular epidemiology and phylogeny inferred from the 7b gene Document date: 2019_8_23
ID: 1s25r3o0_3
Snippet: The complete accessory protein 7b gene (766 nt) was amplified by semi-nested PCR with two rounds of amplification using two pairs of primers previously described by Lin and others [17] . The reaction products of the semi-nested PCR were purified and sequenced by Macrogen Inc., Seoul, Korea. Contigs of the nucleotide sequences were assembled using Phred [12] and Phrap (http://www.phrap.org). The complete 7b gene coding sequences were submitted to .....
Document: The complete accessory protein 7b gene (766 nt) was amplified by semi-nested PCR with two rounds of amplification using two pairs of primers previously described by Lin and others [17] . The reaction products of the semi-nested PCR were purified and sequenced by Macrogen Inc., Seoul, Korea. Contigs of the nucleotide sequences were assembled using Phred [12] and Phrap (http://www.phrap.org). The complete 7b gene coding sequences were submitted to GenBank (JX239089-JX239139).
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