Selected article for: "pcr technique and real time pcr technique"
Author: Osumi, Takafumi; Mitsui, Ikki; Leutenegger, Christian M; Okabe, Ryo; Ide, Kaori; Nishifuji, Koji
Title: First identification of a single amino acid change in the spike protein region of feline coronavirus detected from a coronavirus-associated cutaneous nodule in a cat
  • Document date: 2018_9_20
    • ID: 1o9oqnd6_15
    Snippet: A real-time RT-PCR assay for FCoV is available as a diagnostic technique to provide genetic evidence for FCoVs and their mutated forms in pleural or abdominal effusions obtained from effusive FIP, as well as in pyogranulomatous tissues obtained from non-effusive FIP. 9 This technique enables detection of the 7b gene in any FCoV genotypes and screening for the point mutations M1058L and S1060A in the spike gene. FCoVs with the M1058L mutation have.....
    Document: A real-time RT-PCR assay for FCoV is available as a diagnostic technique to provide genetic evidence for FCoVs and their mutated forms in pleural or abdominal effusions obtained from effusive FIP, as well as in pyogranulomatous tissues obtained from non-effusive FIP. 9 This technique enables detection of the 7b gene in any FCoV genotypes and screening for the point mutations M1058L and S1060A in the spike gene. FCoVs with the M1058L mutation have been identified in 89.9% and 92.0% of cats with effusive and non-effusive FIP, respectively. 7 These mutations are thought to allow FCoVs to acquire macrophage tropism, and thus to spread systemically.

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