Author: Munday, Diane C.; Emmott, Edward; Surtees, Rebecca; Lardeau, Charles-Hugues; Wu, Weining; Duprex, W. Paul; Dove, Brian K.; Barr, John N.; Hiscox, Julian A.
Title: Quantitative Proteomic Analysis of A549 Cells Infected with Human Respiratory Syncytial Virus Document date: 2010_7_20
ID: 2zhaknbi_16
Snippet: Confocal Microscopy-Direct and/or indirect immunofluorescence confocal microscopy images were captured on an LSM510 META microscope (Carl Zeiss Ltd.) equipped with 40Ï« and 63Ï«, 1.4 numerical aperture, oil immersion lenses. Pinholes were set to allow optical sections of 1 m to be acquired. Where possible, all fluorescence was measured in the linear range as the detector is a photomultiplier, and the range indicator was utilized to ensure that no.....
Document: Confocal Microscopy-Direct and/or indirect immunofluorescence confocal microscopy images were captured on an LSM510 META microscope (Carl Zeiss Ltd.) equipped with 40Ï« and 63Ï«, 1.4 numerical aperture, oil immersion lenses. Pinholes were set to allow optical sections of 1 m to be acquired. Where possible, all fluorescence was measured in the linear range as the detector is a photomultiplier, and the range indicator was utilized to ensure that no saturated pixels were obtained on image capture. However, for certain images depending on the distribution of the protein, some parts of the image were outside of the linear range to clearly show the distribution of the appropriate protein. Images were averaged either four or eight times.
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