Quantitative Proteomic Analysis of A549 Cells Infected with Human Respiratory Syncytial Virus

Munday, Diane C.; Emmott, Edward; Surtees, Rebecca; Lardeau, Charles-Hugues; Wu, Weining; Duprex, W. Paul; Dove, Brian K.; Barr, John N.; Hiscox, Julian A.

The following constituents of ND10s were altered in abundance in HRSV-infected cells: the TAR DNA-binding protein (ϳϪ3-fold), eukaryotic translation initiation factor 3 (ϳϪ9fold), and DNA repair protein RAD50 (ϳϪ2.6-fold). ND10 abundance and localization were investigated in HRSV-infected through the use of its major constituent, PML. An antibody that recognized all PML isoforms was applied to both Western blot and indirect immunofluorescence analysis. Western blot analysis indicated that more PML isoforms were present in the nuclear fraction from HRSV-infected cells when compared with mock-infected cells (Fig. 11 ). In the cytoplasmic fraction in HRSV-infected cells, a decreased abundance in the number of PML isoforms was observed when compared with the mock-infected cells (Fig. 11) . The indirect immunofluorescence confocal microscopy data indicated that there were a greater number of ND10s present in the HRSV-infected cells when compared with mock infected-cells (Fig. 12 ). This observation included not only cells that were positively identified as infected but also the bystander cells (Fig. 12 ). This result was in contrast to a previous proteomic anal- FIG. 6