Author: William Binning; Aja E. Hogan-Cann; Diana Yae Sakae; Matthew Maksoud; Valeriy Ostapchenko; Mohammed Al-Onaizi; Sara Matovic; Wei-Yang Lu; Marco A. M. Prado; Wataru Inoue; Vania F. Prado
Title: Chronic hM3Dq signaling in microglia ameliorates neuroinflammation in male mice Document date: 2020_1_28
ID: ely200x3_18
Snippet: Tamoxifen (Sigma, cat#T56648) was dissolved at 100 mg/ml in 10% ethanol/90% corn oil. To induce DREADD expression, tamoxifen (75 mg/kg; i.p.) was injected once per day for 5 consecutive days. For cell culture assays, the tamoxifen active metabolite 4-hydroxytamoxifen (4-OHT, Sigma, cat#H6278) was used instead of tamoxifen. 4-OHT was dissolved in 100% ethanol and used at concentration of 5 µM. Clozapine N-Oxide (CNO, Tocris, cat#4936) was dissolv.....
Document: Tamoxifen (Sigma, cat#T56648) was dissolved at 100 mg/ml in 10% ethanol/90% corn oil. To induce DREADD expression, tamoxifen (75 mg/kg; i.p.) was injected once per day for 5 consecutive days. For cell culture assays, the tamoxifen active metabolite 4-hydroxytamoxifen (4-OHT, Sigma, cat#H6278) was used instead of tamoxifen. 4-OHT was dissolved in 100% ethanol and used at concentration of 5 µM. Clozapine N-Oxide (CNO, Tocris, cat#4936) was dissolved in 0.5% dimethylsulfoxide (DMSO). Calcium dye Rhod-2 AM (Abcam, cat#ab142780) was used at a final concentration of 5 µM in 1% DMSO. Store-operated Ca2+ channel (SOCC) inhibitor SKF-96365 (Santa Cruz, cat#130495-35-1) was diluted in water and used at 20 µM. E. coli 0111:B4 lipopolysaccharide (LPS) (Sigma-Aldrich, cat#L3012, Ec#297-473-0, lot#WI150506) was dissolved in saline and used at a dose of 0.1 mg/kg, i.p. This dose of LPS was chosen because it is the minimum effective dose to elicit pro-inflammatory cytokine response and sickness behavior in mice (Berg et al., 2004) . author/funder. All rights reserved. No reuse allowed without permission.
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