Author: Wang, Yuan; Li, Yan; Ding, Tianbing
Title: Heat shock protein 90ß in the Vero cell membrane binds Japanese encephalitis virus Document date: 2017_6_26
ID: 7cpxg1b4_22
Snippet: HSP90β from the Vero membrane binds JEV on the Vero cell surface. First, western blot analysis was used to test the binding between JEV and HSP90β in the Vero membrane. Following non-reducing SDS-PAGE, Vero membrane protein extract samples were transferred onto PVDF membranes and reacted with rabbit anti-human HSP90β mAb (as anti-monkey HSP90β mAbs are unavailable) and indicated by fluorescent goat anti-rabbit IgG (Fig. 2A, lane 1) . Vero mem.....
Document: HSP90β from the Vero membrane binds JEV on the Vero cell surface. First, western blot analysis was used to test the binding between JEV and HSP90β in the Vero membrane. Following non-reducing SDS-PAGE, Vero membrane protein extract samples were transferred onto PVDF membranes and reacted with rabbit anti-human HSP90β mAb (as anti-monkey HSP90β mAbs are unavailable) and indicated by fluorescent goat anti-rabbit IgG (Fig. 2A, lane 1) . Vero membrane protein samples also reacted alone with JEV and anti-JEV mAb 2H4 (Fig. 2A, lane 2) at the same 90 kDa position, indicating that HSP90β binds JEV. Later, confocal laser scanning microscopy was performed. As shown in Fig. 2B, JEV (green colour, Fig. 2B , panel 2) and HSP90β (red colour, Fig. 2B, panel 3 ) merger displayed yellow fluorescence on the cell surface (Fig. 2B, panel 4) , evidently suggesting that the presence of HSP90β in the cell membrane binds JEV exactly on the cell surface.
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