Heat shock protein 90ß in the Vero cell membrane binds Japanese encephalitis virus

Wang, Yuan; Li, Yan; Ding, Tianbing

When HSP90β-JEV binding was confirmed, an infection inhibition test was conducted using IFA and FCM with a rabbit anti-human HSP90β mAb at various concentrations. In the IFA experiment, the HSP90β-JEV binding clearly decreased with the increasing anti-HSP90β mAb concentrations, and the binding percentages quantified by FCM also exhibited a significant decrease (P<0.01) from 76.17±0.69% (0 µg/ml anti-HSP90β mAb) to 19.15±0.35% (20 µg/ml anti-HSP90β mAb) and further to 10.43±0.53% (100 µg/ml anti-HSP90β mAb) compared with the negative control of 1.70±0.11% (Fig. 3B) . These results clearly demonstrate that the specific anti-HSP90β mAb inhibited HSP90β-JEV binding in a dose-dependent manner. Moreover, it is reasonable to presume that the JEV-binding domain of HSP90β is located at the N-terminus, which is recognised by the anti-HSP90β mAb.