Author: Warnes, Sarah L.; Little, Zoë R.; Keevil, C. William
Title: Human Coronavirus 229E Remains Infectious on Common Touch Surface Materials Document date: 2015_11_10
ID: 4d4l6mzl_33
Snippet: The effect of copper chelators and reactive oxygen species quenchers on infectivity of human coronavirus exposed to copper and copper alloy surfaces. Incorporation of chelators ethylenediaminetetraacetic acid (EDTA) (20 mM) and bathocuproine disulfonic acid (BCS) (20 mM) to chelate Cu(II) and Cu(I), respectively, at the time of inoculation of virus onto the metal surfaces was investigated using a plaque assay. In addition, 20 mM D-mannitol and 20.....
Document: The effect of copper chelators and reactive oxygen species quenchers on infectivity of human coronavirus exposed to copper and copper alloy surfaces. Incorporation of chelators ethylenediaminetetraacetic acid (EDTA) (20 mM) and bathocuproine disulfonic acid (BCS) (20 mM) to chelate Cu(II) and Cu(I), respectively, at the time of inoculation of virus onto the metal surfaces was investigated using a plaque assay. In addition, 20 mM D-mannitol and 20 mM Tiron (4,5-dihydroxy-1,3-benzene disulfonic acid) were used to quench hydroxyl radicals and superoxide, respectively. Stainless steel was used as a control surface and to determine if quenchers and chelators affect viral replication.
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