Author: Baek, Hyekyung; Kim, Kwang Ho; Park, Min Young; Kim, Kyeongryun; Ko, Bokyeong; Seo, Hyung Seok; Kim, Byoung Soo; Hahn, Tae-Wook; Yi, Sun Shin
Title: Establishment of minimal positive-control conditions to ensure brain safety during rapid development of emergency vaccines Document date: 2017_8_22
ID: 52sbckm3_4
Snippet: For treatment, PBS or LPS in PBS were intraperitoneally (i.p.) injected into each mouse (4 mL/kg body weight). At 1 h postinjection (PI) of PBS or LPS, EB dye (2% w/v in PBS, 4 mL/kg, E2129; Sigma) was i.p. injected into each mouse. All mice were sacrificed after 3 h of EB exposure (4 h PI). After sacrifice, blood was collected from heart and serum was separated by using centrifugation. The brain was removed after the mouse had been perfused with.....
Document: For treatment, PBS or LPS in PBS were intraperitoneally (i.p.) injected into each mouse (4 mL/kg body weight). At 1 h postinjection (PI) of PBS or LPS, EB dye (2% w/v in PBS, 4 mL/kg, E2129; Sigma) was i.p. injected into each mouse. All mice were sacrificed after 3 h of EB exposure (4 h PI). After sacrifice, blood was collected from heart and serum was separated by using centrifugation. The brain was removed after the mouse had been perfused with heparinized PBS (0.1 mg/L). Serum and homogenized brain hemisphere of each mouse were dissolved in a 50% (w/v) trichloroacetic acid in PBS solution to eliminate proteins and then subjected to centrifugation at 5,000 × g for 20 min. Supernatant of each sample was diluted 1:3 with ethanol.
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