Author: Baek, Hyekyung; Kim, Kwang Ho; Park, Min Young; Kim, Kyeongryun; Ko, Bokyeong; Seo, Hyung Seok; Kim, Byoung Soo; Hahn, Tae-Wook; Yi, Sun Shin
Title: Establishment of minimal positive-control conditions to ensure brain safety during rapid development of emergency vaccines Document date: 2017_8_22
ID: 52sbckm3_5
Snippet: A spectrophotometer (Infinite F200; Tecan, Switzerland) was used to determine the EB concentrations in the serum and brain hemisphere samples. The fluorescence intensity was measured at 620 ï¬ ex and 680 ï¬ em , and the EB concentration of each sample was calculated according to a standard curve that had been prepared with a range of 10 pg/mL to 10 ng/mL EB in PBS. In order to determine the penetration rate for EB into the BBB, the ratio of EB .....
Document: A spectrophotometer (Infinite F200; Tecan, Switzerland) was used to determine the EB concentrations in the serum and brain hemisphere samples. The fluorescence intensity was measured at 620 ï¬ ex and 680 ï¬ em , and the EB concentration of each sample was calculated according to a standard curve that had been prepared with a range of 10 pg/mL to 10 ng/mL EB in PBS. In order to determine the penetration rate for EB into the BBB, the ratio of EB in brain to EB in blood was calculated. All experiments and procedures were approved by Soonchunhyang University Institutional Animal Care and Use Committee (approval No. SCH17-0024).
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