Author: Baschera, Melinda; Cernela, Nicole; Stevens, Marc J.A.; Liljander, Anne; Jores, Jörg; Corman, Victor Max; Nüesch-Inderbinen, Magdalena; Stephan, Roger
Title: Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels Document date: 2019_3_7
ID: 70exgy3h_15
Snippet: From each plate, individual colonies were picked and suspended in 0.5 ml NaCl 0.85%. Isolates that were confirmed to possess stx (stx1and/or stx2) by real-time PCR were subcultured on sheep blood agar and the presence of stx was confirmed using the Assurance GDS® for Shiga Toxin Genes (Bio Control Systems, Bellevue, WA, USA). From plates yielding more than one stx positive colony, one isolate was randomly chosen for subsequent characterization......
Document: From each plate, individual colonies were picked and suspended in 0.5 ml NaCl 0.85%. Isolates that were confirmed to possess stx (stx1and/or stx2) by real-time PCR were subcultured on sheep blood agar and the presence of stx was confirmed using the Assurance GDS® for Shiga Toxin Genes (Bio Control Systems, Bellevue, WA, USA). From plates yielding more than one stx positive colony, one isolate was randomly chosen for subsequent characterization.
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