Author: Strating, Jeroen R.P.M.; van der Linden, Lonneke; Albulescu, Lucian; Bigay, Joëlle; Arita, Minetaro; Delang, Leen; Leyssen, Pieter; van der Schaar, Hilde M.; Lanke, Kjerstin H.W.; Thibaut, Hendrik Jan; Ulferts, Rachel; Drin, Guillaume; Schlinck, Nina; Wubbolts, Richard W.; Sever, Navdar; Head, Sarah A.; Liu, Jun O.; Beachy, Philip A.; De Matteis, Maria A.; Shair, Matthew D.; Olkkonen, Vesa M.; Neyts, Johan; van Kuppeveld, Frank J.M.
Title: ITRACONAZOLE INHIBITS ENTEROVIRUS REPLICATION BY TARGETING THE OXYSTEROL-BINDING PROTEIN Document date: 2015_1_29
ID: 3kmqy07w_4
Snippet: Subconfluent monolayers of cells seeded in 96-well plates were infected with virus at the indicated MOI. After 30 min incubation at 37°C, the virus was removed and fresh (compound-containing) medium was added after which the cells were incubated at 37°C for the indicated length of time. For the measurement of infectious virus particles, virus was released from the cells by three rounds of freeze-thawing and virus titers were determined by end-p.....
Document: Subconfluent monolayers of cells seeded in 96-well plates were infected with virus at the indicated MOI. After 30 min incubation at 37°C, the virus was removed and fresh (compound-containing) medium was added after which the cells were incubated at 37°C for the indicated length of time. For the measurement of infectious virus particles, virus was released from the cells by three rounds of freeze-thawing and virus titers were determined by end-point dilution assay. In the case of infections with RLuc-CVB3 or RLuc-EMCV, cells were lysed at 6-7hr p.i. and Renilla luciferase activity was measured with the Renilla Luciferase Assay System (Promega) according to the manufacturer's instructions. Where indicated, a cell viability MTS assay was performed in parallel as described above.
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