Title: The first membrane spanning region of the lamin B receptor is sufficient for sorting to the inner nuclear membrane Document date: 1993_2_1
ID: 3qi2llmr_25
Snippet: The primary structure of LBR (depicted schematically in Fig. 1 A) was previously derived from overlapping cDNA clones. We constructed a full-length cDNA (see Materials and Methods), expressed the protein in a cell-free reticulocyte lysate system ( Fig. 1 B) , and immunoprecipitated the protein with the previously characterized guinea pig antibodies against turkey LBR (Worman et al., 1988) (Fig. 1 C) . Like its in vivo synthesized counterpart the .....
Document: The primary structure of LBR (depicted schematically in Fig. 1 A) was previously derived from overlapping cDNA clones. We constructed a full-length cDNA (see Materials and Methods), expressed the protein in a cell-free reticulocyte lysate system ( Fig. 1 B) , and immunoprecipitated the protein with the previously characterized guinea pig antibodies against turkey LBR (Worman et al., 1988) (Fig. 1 C) . Like its in vivo synthesized counterpart the in vitro expressed LBR migrated faster than expected, as a protein of 58 kD rather than the expected 73 kD.
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