Selected article for: "correct orientation and early promoter respect"
Title: The first membrane spanning region of the lamin B receptor is sufficient for sorting to the inner nuclear membrane
  • Document date: 1993_2_1
    • ID: 3qi2llmr_7
    Snippet: p38ATMI-R p58cDNA was digested with Hinfl, blunt ended with DNA polymerase, Klenow fragment, and digested with EcoRL The resulting fragment (containing the 5' untranslated region and codons 1 through 206) was cloned into the EcoRI and Smal sites of the pSVX polylinker in the correct orientation with respect to the SV-40 early promoter and the HA tag was inserted exactly as described for p58ATM2-8, p58ATM1-8 contains p58 amino acids 1-206......
    Document: p38ATMI-R p58cDNA was digested with Hinfl, blunt ended with DNA polymerase, Klenow fragment, and digested with EcoRL The resulting fragment (containing the 5' untranslated region and codons 1 through 206) was cloned into the EcoRI and Smal sites of the pSVX polylinker in the correct orientation with respect to the SV-40 early promoter and the HA tag was inserted exactly as described for p58ATM2-8, p58ATM1-8 contains p58 amino acids 1-206.

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