Author: Qiao, Hui; Pelletier, Sandra L.; Hoffman, Lucas; Hacker, Jill; Armstrong, R. Todd; White, Judith M.
Title: Specific Single or Double Proline Substitutions in the “Spring-loaded” Coiled-Coil Region of the Influenza Hemagglutinin Impair or Abolish Membrane Fusion Activity Document date: 1998_6_15
ID: 78fjem8s_14
Snippet: Cells expressing wt-or mutant HAs were washed, lysed in a cell lysis buffer containing 1% NP-40 and protease inhibitors, and immunoprecipitated essentially as described (Kemble et al., 1993) . Modifications were that lysis was conducted at RT for 15 min and that the amount of the site A monoclonal antibody (gift of Dr. J. Skehel, Medical Research Council, Mill Hill, England) was decreased to 0.1-0.3 g/ml......
Document: Cells expressing wt-or mutant HAs were washed, lysed in a cell lysis buffer containing 1% NP-40 and protease inhibitors, and immunoprecipitated essentially as described (Kemble et al., 1993) . Modifications were that lysis was conducted at RT for 15 min and that the amount of the site A monoclonal antibody (gift of Dr. J. Skehel, Medical Research Council, Mill Hill, England) was decreased to 0.1-0.3 g/ml.
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