Selected article for: "base calling and dna sequence"
Author: Lee, Charlie Wah Heng; Koh, Chee Wee; Chan, Yang Sun; Aw, Pauline Poh Kim; Loh, Kuan Hon; Han, Bing Ling; Thien, Pei Ling; Nai, Geraldine Yi Wen; Hibberd, Martin L.; Wong, Christopher W.; Sung, Wing-Kin
Title: Large-scale evolutionary surveillance of the 2009 H1N1 influenza A virus using resequencing arrays
  • Document date: 2010_2_25
    • ID: 1rhy8td0_5
    Snippet: To address if these arrays can be used as a practical, large-scale re-sequencing tool, we have developed a system comprising customized sequence amplification primers, a 12-plex DNA resequencing array and an automated base-calling and variant analysis software (EvolSTAR). We demonstrate that the sequences obtained from the array are highly reproducible with !99.99% accuracy and 99.02 ± 0.82% genome coverage. The short turnaround time from sample.....
    Document: To address if these arrays can be used as a practical, large-scale re-sequencing tool, we have developed a system comprising customized sequence amplification primers, a 12-plex DNA resequencing array and an automated base-calling and variant analysis software (EvolSTAR). We demonstrate that the sequences obtained from the array are highly reproducible with !99.99% accuracy and 99.02 ± 0.82% genome coverage. The short turnaround time from sample to sequence and analysis results ($30 h for 24 samples) makes this kit an efficient large-scale evolutionary surveillance tool.

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