Selected article for: "DENV MINI RNA and flip PK1 Flip mutant"
Author: Sztuba-Solinska, Joanna; Teramoto, Tadahisa; Rausch, Jason W.; Shapiro, Bruce A.; Padmanabhan, Radhakrishnan; Le Grice, Stuart F. J.
Title: Structural complexity of Dengue virus untranslated regions: cis-acting RNA motifs and pseudoknot interactions modulating functionality of the viral genome
  • Document date: 2013_3_26
    • ID: 1pbd4maf_37
    Snippet: We also investigated how introducing 3-nt compensatory mutations within the 5 0 CS (U141-C144) to restore the 5 0 -3 0 CS in the presence of 'flipped' TL2 and PK1 affected DENV MINI RNA structure. SHAPE analysis of mutant TL2 Flip PK1 Flip -3 predicted almost complete reversion to the wild-type secondary structure ( Figure 6 and Supplementary Figures S13 and S14) . The only rearrangement occurred within the 5 0 -3 0 CS region, which was shortened.....
    Document: We also investigated how introducing 3-nt compensatory mutations within the 5 0 CS (U141-C144) to restore the 5 0 -3 0 CS in the presence of 'flipped' TL2 and PK1 affected DENV MINI RNA structure. SHAPE analysis of mutant TL2 Flip PK1 Flip -3 predicted almost complete reversion to the wild-type secondary structure ( Figure 6 and Supplementary Figures S13 and S14) . The only rearrangement occurred within the 5 0 -3 0 CS region, which was shortened from 11-nt helical region to 6-nt stem (C135-U140 and A624-A618), releasing PK1 (C613-A617) and compensatory residues into single-stranded loops. Thus, the complementarity and single-strand character of both the downstream component of 5 0 CS and PK1 proved insufficient to maintain their base pairing interaction. Pb 2+ -induced hydrolysis of TL2 Flip PK1 Flip -3 mutant ( Figure 6 insert and Supplementary Figure 15 ) indicated moderate cleavage within the region carrying compensatory mutations, as well as within PK1 (A615-C616).

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