Selected article for: "protein expression and recombinant protein expression"
Author: Uversky, Vladimir N
Title: The alphabet of intrinsic disorder: II. Various roles of glutamic acid in ordered and intrinsically disordered proteins
  • Document date: 2013_4_1
    • ID: 63gh2tg4_39
    Snippet: PPE antigens. Proline and glutamic acid rich proteins (or PPErepeat containing proteins, or PPE proteins) are important T-cell antigens produced by Mycobacterium avium subsp Paratuberculosis (Map). 237 One of the PPEs is a 34.9 kDa protein (359 residues, pI 4.31) which following recombinant expression in E. coli was shown to elicit significant delayed type hypersensitivity skin reaction in mice sensitized with Map, suggesting that this recombinan.....
    Document: PPE antigens. Proline and glutamic acid rich proteins (or PPErepeat containing proteins, or PPE proteins) are important T-cell antigens produced by Mycobacterium avium subsp Paratuberculosis (Map). 237 One of the PPEs is a 34.9 kDa protein (359 residues, pI 4.31) which following recombinant expression in E. coli was shown to elicit significant delayed type hypersensitivity skin reaction in mice sensitized with Map, suggesting that this recombinant PPE protein of Map was definitely associated with cellular immune response. 237 Curiously, this PPE contains 73 alanines, 44 glycines, 37 prolines, 20 aspartic acids but just 10 glutamic acids.

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