The amino-terminal domain of the lamin B receptor is a nuclear envelope targeting signal

Plasmid LMBR-CHL was constructed to express the first 191 amino acids of the chicken LBR followed by the transmembrane domain and portion of the carboxy-terminal domain of chicken hepatic leetin. DNA from nucleotide 183 to nucleotide 510 of chicken hepatic lectin eDNA that coded for amino acids 24 to 131 (numbering according to Mellow et al., 1988) was amplified by PCR using the pSP64 clone as template. The sense primer used in this reaction had a BspE1 engineered into its 5' end and the antisense primer a complementary stop codon preceded by an ApaI site at its 5' end. The product was purified from the reaction mixture by phenol:chloroform extraction, digested with BspE1 and ApaI, purified again and ligated into plasmid LMBR that was digested with BspE1 and ApaI (LBR eDNA has a BspE1 restriction site at nucleotide +567).