Author: Mao, Changyi; Yu, Zhihui; Li, Chengliang; Jin, Yongguo; Ma, Meihu
Title: The Functional Properties of Preserved Eggs: From Anti-cancer and Anti-inflammatory Aspects Document date: 2018_7_31
ID: 3eeykk89_27
Snippet: Approximately 2.5×10 5 cells/well of Caco-2 cells were placed in 6-well plates and incubated for 24 h. The cells were exposed to 2.5, 5, 10 mg/mL of the GI digests for 24 h after carefully washed with PBS, set the cell without the digests as a blank control. After incubation, the cells were immediately collected and washed 2 times by PBS, then centrifuged at a speed of 2,000×g for 5 min to remove the supernatant, 500 μL binding buffer, 5 μL A.....
Document: Approximately 2.5×10 5 cells/well of Caco-2 cells were placed in 6-well plates and incubated for 24 h. The cells were exposed to 2.5, 5, 10 mg/mL of the GI digests for 24 h after carefully washed with PBS, set the cell without the digests as a blank control. After incubation, the cells were immediately collected and washed 2 times by PBS, then centrifuged at a speed of 2,000×g for 5 min to remove the supernatant, 500 μL binding buffer, 5 μL Annexin V-FITC and PI were added to suspend the cells, respectively. Mixtures were finally incubated in dark at room temperature for 15 min prior to investigation by flow cytometry (FACSCalibur, BD Co., Ltd, USA) for 1 h.
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