Selected article for: "Acid kit and Nucleic acid"

Author: Chen, Jing; Li, Xiaoguang; Wang, Wei; Jia, Ying; Lin, Fei; Xu, Jie
Title: The prevalence of respiratory pathogens in adults with community-acquired pneumonia in an outpatient cohort
  • Document date: 2019_7_30
  • ID: 6ghxcphh_10
    Snippet: 3. Results determination: The following conditions should be simultaneously met: 1. sleek S-Curve; 2. CT ≤35 3; 3. ΔRn>1×10 4 . 4. Limits of detection for the different pathogens: In the detection kit for the respiratory viruses, when the viral nucleic acid <10 3 in the reaction system, it may not be detected. Although the target sequences are in the highly conserved domain, the viral genes are always mutating, which can lead to false negat.....
    Document: 3. Results determination: The following conditions should be simultaneously met: 1. sleek S-Curve; 2. CT ≤35 3; 3. ΔRn>1×10 4 . 4. Limits of detection for the different pathogens: In the detection kit for the respiratory viruses, when the viral nucleic acid <10 3 in the reaction system, it may not be detected. Although the target sequences are in the highly conserved domain, the viral genes are always mutating, which can lead to false negatives.In the detection kit for the respiratory bacteria, when the bacterial nucleic acid <10 2 in the reaction system, it may not be detected. The target sequences are in the highly conserved domain; if the bacterial nucleic acid mutates in the target position, it may lead to false negatives.

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