Selected article for: "citrate buffer and ph citrate buffer"
Title: The rubella virus E1 glycoprotein is arrested in a novel post-ER, pre- Golgi compartment
  • Document date: 1992_8_2
    • ID: 04455ffs_17
    Snippet: For endo H digestions, immunoprecipitates were released from the protein A Sepharose beads by heating in 0.5% SDS, 100 mM sodium citrate (pH 5.5) at 100*C for 5 min. The eluates were diluted with an equal volume of 100 mM sodium citrate buffer (pH 5.5) and incubated with or without 2.5 mU endo H for at least 8 h at 37~ (19) . For endo D digestions, samples were adjusted to 50 mM sodium citrate (pH 6.5), 10 mM EDTA, 2% Triton X-100, 0.25% SDS, and.....
    Document: For endo H digestions, immunoprecipitates were released from the protein A Sepharose beads by heating in 0.5% SDS, 100 mM sodium citrate (pH 5.5) at 100*C for 5 min. The eluates were diluted with an equal volume of 100 mM sodium citrate buffer (pH 5.5) and incubated with or without 2.5 mU endo H for at least 8 h at 37~ (19) . For endo D digestions, samples were adjusted to 50 mM sodium citrate (pH 6.5), 10 mM EDTA, 2% Triton X-100, 0.25% SDS, and incubated with 2.5 mU endo D for at least 8 h at 37~ as described (63) .

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